“Tween Egg Medium” for Isolating Mycobacteria from Sputum Specimens
نویسندگان
چکیده
منابع مشابه
PKO – Alternative method for isolating mycobacteria from sputum
We elaborated an alternative culture method, which we denominated PKO (initials in tribute of respect to Petroff, Kudoh and Ogawa), for isolating Mycobacterium tuberculosis from sputum for diagnosis of pulmonary tuberculosis (TB), and to compare its performance with the Swab and Petroff methods. For the technique validation, sputum samples from patients suspected of pulmonary TB cases were exam...
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Over a two-year period, 2949 non-sputum specimens were cultured on two slopes of Löwenstein-Jensen (LJ) medium, two bottles of Kirchner liquid medium, made selective by adding polymyxin B, carbenicillin, trimethoprim and amphotericin B, and a selective 7H11 agar slope. Pus and CSF were inoculated into this set without prior treatment, but other specimens were decontaminated with sulphuric acid....
متن کاملCulture of specimens other than sputum for Mycobacteria.
A comparison has been made of Lowenstein-Jensen medium, with or without added pyruvate, 7H11 oleic acid-albumin agar slopes made selective by the addition of antibiotics, and selective Kirchner medium in the isolation of tubercle bacilli from specimens other than sputum. A combination of a standard mild preliminary treatment with sulphuric acid and selective medium reduced the contamination rat...
متن کاملTwo liquid medium systems, mycobacteria growth indicator tube and MB redox tube, for Mycobacterium tuberculosis isolation from sputum specimens.
Two manual liquid medium systems, the Mycobacteria Growth Indicator Tube (MGIT) and MB Redox tube systems, were evaluated in comparison to the radiometric BACTEC-460 semiautomated system for recovery of Mycobacterium tuberculosis from sputum specimens. The highest level of recovery, from a total of 77 culture-positive specimens, occurred with the BACTEC-460 system (92.2%), followed by the MB Re...
متن کاملIsolation of mycobacteria from undecontaminated specimens with selective 7H10 medium.
Media containing antimicrobial agents have been formulated for use as an adjunct to the standard media in an effort to reduce contamination and improve isolation of mycobacteria from clinical specimens. Selective 7H10 (S7H10) was developed for use in the isolation of mycobacteria from undecontaminated material. During a 33-month period, 10,782 clinical specimens were cultured in parallel on S7H...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 1979
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.1979.tb02816.x